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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Cell Calcium. 2013 Mar 13;53(0):315–326. doi: 10.1016/j.ceca.2013.02.001

FIGURE 4. Characteristics and release properties of 6 wk old WT and Trpc1−/− BMMCs.

FIGURE 4

A: Flow cytometry conducted on 7 wk old BMMCs prepared from WT and Trpc1−/− mice showed an identical expression of FcεRI and KIT. B: Toluidine blue staining of 6 wk old cultures of WT (left) and Trpc1−/− (right) BMMCS show identical granularity. C: β-hexosaminidase release in 6 wk old BMMC cultures prepared from WT and Trpc1−/− show identical degranulation. Anti-DNP-IgE-sensitized cells were treated with increasing concentrations of antigen (Ag) (C) or Ag and SCF (30 ng/ml) (D) for 30 min. The data are presented as the mean ± S.E. of (n=4) separate experiments conducted in duplicate. Production of TNF-α (E), GM-CSF (F), and IL-6 (G) were elevated in Trpc1−/− BMMCs. IgE sensitized cells were incubated with antigen (10 ng/ml) and SCF (10 ng/ml) individually or in combination for 6h. Values for TNF-α and IL-6 are presented as the mean ± SEM from 8–10 independent experiments. For GM-SCF, values are presented as the mean ± SEM from 3 independent experiments. (*p<0.05, **p<0.01,*** p<0.001, two tailed Student’s t-test).