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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Cell Calcium. 2013 Mar 13;53(0):315–326. doi: 10.1016/j.ceca.2013.02.001

FIGURE 6. Calcium signaling and mediator release in Trpc6−/− and Trpc1−/−/Trpc6−/− BMMCs.

FIGURE 6

A–D: Changes in cytosolic calcium in response to DNP-HSA (Ag; 10 ng/ml)) (A,C) and thapsigargin (thaps: 800 nM) (B,D) in sensitized WT and Trpc1−/− (A,B) and WT and Trpc1−/−/Trpc6−/− BMMCs (C,D) E: DNP-HSA (Ag)-mediated degranulation in 6 week old sensitized BMMC cultures prepared from WT and Trpc1−/−/Trpc6−/− BMMCs. The data in A to E are presented as means ± S.E of (n=2–3) separate experiments conducted in duplicate. F: Release of TNF-α from sensitized Trpc1−/−/Trpc6−/− BMMCs. IgE-sensitized cells were incubated with antigen (10 ng/ml) and SCF (10 ng/ml) individually or in combination for 6h. The level of TNF-α was determined using ELISA. Values are presented as the mean ± SEM from 4 independent experiments. *p<0.05 for comparison with Ag/SCF G: Mice were sensitized using 3 μg anti-DNP IgE mAb. After 24 h mice received an injection of 200 μg DNP-HSA, and the change in core body temperature was monitored for 2 hours. Data is represented as the mean ±SEM. (*p<0.05, ** p<0.01). N=4.