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Effect of TRPC1 deficiency on phoshorylation of the calcium-regulated transcription factors JUN (A,B) or NFAT (B,D). In C and D, the blots in A and B were respectively scanned to quantitate. These values were then normalized to the responses obtained with Ag alone in the WT cells. The blots in A and B are representative of 6–7 independent experiments. The data in B and D are presented as the mean +/− SEM of these data. **p<0.01, **P<0.001, 2 tailed Student’s t-test).
