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. 2013 May 10;3:21. doi: 10.1186/2045-3701-3-21

Table 2.

Ligation program in Golden Gate cloning round 1

Cycle number Temperature (°C) Time (min)
1-15
 37
 5
 
 16
 10
16
 16
 15
17
 50
 10
18 80 5