Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Ligation mix in Golden Gate cloning round 2
| Components | pCS2-TALEN Mix (μL) |
|---|---|
| Buffer 3 (NEB) |
1 |
| pFUS_A-RVDs vectors (150 ng/μL) |
0.8 |
| pFUS_B-RVDs vectors (150 ng/μL) |
0.8 |
| Last Repeats vectors (150 ng/μL) |
0.6 |
| BsmbI (NEB) |
0.8 |
| pCS2-TALEN-ELD/KKR vectors (150 ng/μL) |
1 |
| dH2O |
5 |
| Total | 10 |
