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PCR mix for amplification of target genomic DNA
| Components | PCR Mix (μL) |
|---|---|
| 5 × GoTaq Buffer |
5 |
| 25 mM MgCl2 |
2.5 |
| 10 mM dNTP Mix (Fermentas) |
0.5 |
| Primer 1/2 (10 mM) |
0.5 |
| Primer 3 (10 mM) |
0.5 |
| Genomic DNA |
0.5 |
| GoTaq DNA polymerase (Promega) |
0.5 |
| dH2O |
15 |
| Total | 25 |
