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. 2013 May 10;8:6. doi: 10.1186/1750-2187-8-6

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Copyright © 2013 Li et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Stable over-expression of OGR1 inhibited MCF7 cell migration in vitro. (A) Identification of MCF7 clones stably over-expressing OGR1 (left panel) or GPR4 (right panel) by real-time RT-PCR. (B) The motility of each cell clone was assessed by wound-healing assays. Cells migrated were monitored every hour in a Multi-Dimensional Workstation for Live Cell Imaging (Carl Zeiss). (C) Cell migration was analyzed using transwell assays (left). Representative images of cell migrated to the bottom of the inserts of the control cells (MCF7, MCF7-pHM6), MCF7-OGR1, or MCF7-GPR4 cells (left) and the mean percentage of cells migrated (right) are shown. ** P<0.01. Data are representative of three independent experiments.