Figure 1. Conditional deletion of locus encoding transcription factor Zfp148.

(A) Schematic representation of mouse ZBP-89 locus (Zfp148) and strategy for gene targeting. Intestine specific deletion was achieved by breeding to the VillinCre line (ZBP-89^ΔInt). (B) PCR Genotyping. Lane 1= DNA markers, lane 2 = wild-type (+/+) tail DNA, lane 3 = targeted locus, lanes 4–6, intestinal mucosa where Cre is active. ZBP-89 expression in the colon of WT and ZBP-89^ΔInt mice detected by (C) Western blot of ZBP-89 normalized to β-actin from after colonic epithelium separated by chelation. (D) Immunofluorescence. FITC label = ZBP-89, DAPI = nuclei. Arrows indicate residual ZBP-89 nuclear staining in the lamina propria/smooth muscle layer.