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(A) Phase contrast microscopy images of four ATC cell lines cultured as monolayers in RPMI medium. (B) Growth proliferation curve over 96 hours demonstrates the proliferative potential of these ATC cell lines. (C) qRT-PCT analysis of thyroid transcription factors Pax8 and TTF1 and thyroid differentiation markers TSHR, TG, NIS, and TPO. Human GAPDH was used as the housekeeping gene during the amplifications.
