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. 2013 May 28;8(5):e65095. doi: 10.1371/journal.pone.0065095

Figure 2. ATC cells retain self-renewal capacity in vitro.

Figure 2

(A) Representative phase contrast microscopy analysis of colonies (left) and thyrospheres (right) after seven days of culture. Scale bar, 100 µm. (B) Percentage of primary and secondary thyrospheres in all four ATC cell lines. (C) Representative confocal microscopy images of THJ-21T thyrospheres indicated the expression of Nanog (red), Oct4 (green) and DAPI (blue). Scale bar, 10 µm (upper panel). Representative phase contrast images of THJ-21T parental monolayer cells indicated the expression of Nanog and Oct4 is unique to thyrospheres and is not seen in the parental monolayer cells (lower panel).