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. 2013 May 28;8(5):e65031. doi: 10.1371/journal.pone.0065031

Table 1. Primer sequences used for real-time RT-qPCR.

Gene Genebank ID Amplicon Size Forward Sequence Reverse Sequence Span Intron PCR Efficiency
Cxcl1 (Kc) NM_008176 101 bp TCTCCGTTACTTGGGGACAC CCACACTCAAGAATGGTCGC Yes 103%
Cxcl2 (Mip2a) NM_009140 102 bp AACATCCAGAGCTTGAGTGTGA TTCAGGGTCAAGGCAAACTT Yes 100%
Il6 NM_031168 139 bp ATGGATGCTACCAAACTGGAT TGAAGGACTCTGGCTTTGTCT Yes 103%
Ifng NM_008337 90 bp TGAGCTCATTGAATGCTTGG ACAGCAAGGCGAAAAAGGAT Yes 97%
Ccl2 (Mcp1) NM_011333 108 bp ATTGGGATCATCTTGCTGGT CCTGCTGTTCACAGTTGCC Yes 103%
Ccl3 (Mip1a) NM_011337 91 bp GTGGAATCTTCCGGCTGTAG ACCATGACACTCTGCAACCA Yes 95%
Ccl4 (Mip1b) NM_013652 109 bp GAAACAGCAGGAAGTGGGAG CATGAAGCTCTGCGTGTCTG Yes 95%
Tnf NM_013693 103 bp AGGGTCTGGGCCATAGAACT CCACCACGCTCTTCTGTCTAC Yes 99%
Actinb NM_007393 170 bp CCAACCGTGAAAAGATGACC AGCATAGCCCTCGTAGATG Yes 98%
B2m NM_009735 110 bp ATGGCTCGCTCGGTGACCCT TTCTCCGGTGGGTGGCGTGA Yes 99%