Table 1. Primer sequences used for real-time RT-qPCR.
Gene | Genebank ID | Amplicon Size | Forward Sequence | Reverse Sequence | Span Intron | PCR Efficiency |
Cxcl1 (Kc) | NM_008176 | 101 bp | TCTCCGTTACTTGGGGACAC | CCACACTCAAGAATGGTCGC | Yes | 103% |
Cxcl2 (Mip2a) | NM_009140 | 102 bp | AACATCCAGAGCTTGAGTGTGA | TTCAGGGTCAAGGCAAACTT | Yes | 100% |
Il6 | NM_031168 | 139 bp | ATGGATGCTACCAAACTGGAT | TGAAGGACTCTGGCTTTGTCT | Yes | 103% |
Ifng | NM_008337 | 90 bp | TGAGCTCATTGAATGCTTGG | ACAGCAAGGCGAAAAAGGAT | Yes | 97% |
Ccl2 (Mcp1) | NM_011333 | 108 bp | ATTGGGATCATCTTGCTGGT | CCTGCTGTTCACAGTTGCC | Yes | 103% |
Ccl3 (Mip1a) | NM_011337 | 91 bp | GTGGAATCTTCCGGCTGTAG | ACCATGACACTCTGCAACCA | Yes | 95% |
Ccl4 (Mip1b) | NM_013652 | 109 bp | GAAACAGCAGGAAGTGGGAG | CATGAAGCTCTGCGTGTCTG | Yes | 95% |
Tnf | NM_013693 | 103 bp | AGGGTCTGGGCCATAGAACT | CCACCACGCTCTTCTGTCTAC | Yes | 99% |
Actinb | NM_007393 | 170 bp | CCAACCGTGAAAAGATGACC | AGCATAGCCCTCGTAGATG | Yes | 98% |
B2m | NM_009735 | 110 bp | ATGGCTCGCTCGGTGACCCT | TTCTCCGGTGGGTGGCGTGA | Yes | 99% |