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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Cancer Lett. 2013 Feb 10;335(1):41–51. doi: 10.1016/j.canlet.2013.01.054

Figure 2.

Figure 2

PF-03084014 monotherapy and combination of PF-03084014 with GEM is highly effective in reducing putative pancreatic cancer stem cells. A) Flow cytometry of tumor cells sorted from Panc215 (after 4 weeks of treatment). GEM treatment was not capable of diminishing the CSC pool as compared to the vehicle treated mice. However, PF-03084014 alone, and combination of PF-03084014 with GEM decreased in CD24+CD44+ tumor cells compared with GEM treatment (3.72 and 3.04-fold, respectively). B) Representative photomicrographs of ALDH staining, indicating that PF-03084014 and combination of PF-03084014 with GEM is highly effective in reducing ALDH+ cells. Both ALDH+ and ALDH- tumor cells were counted in 5 random high power fields of 2 tumors each from treatment arms and expressed as % ALDH+ cells. Arrowheads point towards ALDH+ cells. PF-03084014 alone, and combination of PF-03084014 with GEM decreased in ALDH+ tumor cells compared with GEM treatment (3.4 and 3.7-fold, respectively). Numbers inside the figures shows the average (%) of positively stained cells.