Table 1. Performance characteristics of a multiplexed MRM-MS assay.

Isotope labeled apoA-I was used as the internal standard for all six proteins in the assay and correlation with immunoassay was determined along with linearity and imprecision.

			Imprecision
	Correlation with Immunoassay (N=30)	Linearity	LC-MS (%CV)	Digestion (%CV)	Overall (%CV)
A-I	0.95	0.9996	1.15%	0.25%	1.18%
B	0.61	0.9997	4.59%	4.43%	6.38%
C-II	0.92	0.9948	8.38%	7.99%	11.58%
C-III	0.88	0.9976	8.39%	4.41%	9.48%
E	0.92	0.9980	6.03%	4.93%	7.79%
J	0.79	0.9996	9.51%	7.43%	12.07%