Figure 2. Circulating tumor cells have increased tumorigenicity.

1×10⁶ BNL 1ME A.7R.1 or OL0825 cells were subcutaneously implanted into 7 separate Balb/c mice or surgically implanted into the liver of 3 separate Balb/c mice. A. Tumors from OL0825 had significantly greater volume than those from BNL 1ME A.7R.1. B. OL0825 cells resulted in tumor development twice as fast as BNL 1ME A.7R.1 cells in the survival surgical intrahepatic implantation model. C. Representative images of orthotopic tumor in the liver and metastatic lesions in the lung from implantation of OL0825 into liver of immune competent wild type Balb/c mice. Images of H&E-stained slides were acquired at ×40 (objective lens). Results presented as mean ± SEM; P<0.01.