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. 2013 May 28;8(5):e63765. doi: 10.1371/journal.pone.0063765

Figure 3. Circulating tumor cells have increased mesenchymal characteristics.

Figure 3

Analysis of gene and protein expression of markers of epithelial-mesenchymal transition were performed using qPCR and Western blotting respectively. There is significantly decreased E-cadherin gene expression (A), increased fibronectin gene expression (B), increased collagen I gene expression (C) and increased vimentin gene expression (D) by OL0825 than BNL 1ME A.7R.1 cells. E. OL0825 demonstrate increased fibronectin, collagen I, vimentin and decreased E-cadherin protein expression in comparison to BNL 1ME A.7R.1 cells. Relative densitometry is indicated below the bands. Results presented as mean ± SEM; P<0.05; P<0.01; N = 3.