Figure 5. HGF induces EMT in BNL 1ME A.7R.1 cells.

BNL 1ME A.7R.1 cells were cultured in 6-well plates and treated with 25 ng/ml mouse HGF (R & D Systems) for 5 days. Control cells were untreated. RNA and protein extraction were performed and qPCR and Western blotting were performed as described in “Experimental procedures” section. HGF-treated BNL 1ME A.7R.1 cells demonstrated increased fibronectin gene and protein expression (A, D), increased collagen I gene and protein expression (B, D) and loss of E-cadherin gene and protein expression (C, D). Relative densitometry is indicated below the bands (D). Results presented as mean ± SEM; P<0.01; N = 3.