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. 2013 May 28;8(5):e64535. doi: 10.1371/journal.pone.0064535

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© 2013 Liu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Histone H3 methylation changes at the promoter regions of genes of interest (GoI) in SW480 cells. Chromatin from SW480 cells was harvested after 5 h of 10-µM PEITC treatment. The results of ChIP analyses using (A) anti-trimethyl-Histone H3 Lys27 (H3K27me3) and (B) anti-dimethyl-Histone H3 Lys9 (H3K9me2) antibodies are shown. Out of 13 GoIs tested in SW480 cells, six genes showed statistically significant PEITC-associated changes in H3K27me3 states and one gene showed statistically significant PEITC-associated changes in H3K9me2 state. DNA sequences were quantified by real-time PCR using promoter primers. Average percentage input ± SEM (n = 3) from each experiment is plotted. *p<0.05, **p<0.01, ***p<0.001 compared with positive-control cells.