Table 4. Time-dependent correlation between mRNA expression and H3 modifications.

Gene Name	Effects	10 µM PEITC treatment at four time points: 5, 8, 12, 18 h
		Observation summary (significance compared with control)
STAT1	Relative mRNA H3K27me3 (%)	Levels decreased in a time-dependent manner up to 12 h Levels increased in a time-dependent manner up to 12 h; correlate with mRNA expression (this data is shown as a representative histogram in Figure 5 in main text; while remaining data presented in this table are shown as Figures S1, S2, S3, S4, S5)
NFκB1	Relative mRNA	Suppression of mRNA only at 5 h; for 8 h onward, no significant suppression
	H3K27me3 (%)	With increase of mRNA levels 8h onward, H3K27me3 levels decreased and lost significance; correlates with mRNA expression (Figure S1)
MMP7	Relative mRNA	Levels decreased in a time-dependent manner up to 18 h
	H3K27me3 (%)	No correlation with mRNA levels except at 5 h. H3K27me3 and mRNA levels both decreased at later time points (Figure S2)
	H3Ac (%)	Levels decreased in a time-dependent manner up to 18 h; correlate with mRNA expression (Figure S2)
CCL2	Relative mRNA	Expression levels significantly low at all time points;
	H3K27me3 (%)	Correlation with mRNA unlikely (Figure S4)
CD40	Relative mRNA	Expression levels significantly low at all time points;
	H3K27me3 (%)	Changes do not correlate with mRNA levels over time (Figure S3)
	H3K9me2 (%)	Changes do not correlate with mRNA levels over time (Figure S3)
IL8	Relative mRNA	Suppression of mRNA only at 5 h; for 8 h onward, no significant suppression
	H3K27me3 (%)	With increase of mRNA levels, H3K27me3 level decreased, but remained significantly higher than control; partial correlation with mRNA levels likely (Figure S5)
	H3Ac (%)	Acetylation levels remained low even after mRNA suppression was lost at later time points. No time-dependent correlation with mRNA levels (Figure S5)

Effects: mRNA levels are normalized to GAPDH levels and quantified relative to positive control; ChIP values are determined as the percentage of input; *p<0.05 is considered significant.

H3K27me3: trimethylation of histone H3 at lysine 27; H3K9me2: dimethylation of histone H3 at lysine 9; both marks were expected to have an inverse correlation with mRNA expression; H3Ac: acetylation of histone H3; this mark was expected to have a direct correlation with mRNA expression; all six listed genes were tested for changes in H3K27me3, H3k9me2, and H3-Ac changes in their promoter regions. Only statistically significant differential observations relative to positive control (LPS-induced but not treated with PEITC) are noted in the table and discussed in the manuscript.