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. 2013 May 28;8(5):e63848. doi: 10.1371/journal.pone.0063848

Figure 7. Distribution of GFAP in surface dividing VZ precursor cells during M-phase.

Figure 7

Mitotic cells at the ventricular surface of the macaque occipital neocortex at E65 ( A–C ) and E80 ( D ) immunostained for the mitotic cell marker 4A4 (green) and GFAP (red), and costained with DAPI nuclear stain (blue). ( A ) 4A4+/GFAP+ prophase cell in the E65 macaque occipital cortex. During prophase there was strong GFAP immunoreactivity (IR) at the apical and basal poles of the cell. GFAP-IR often extended from the soma into the initial segment of the pial fiber on the basal pole of the cell to give the appearance of a “GFAP cap” (white arrowheads), and also labeled the pial fiber (white arrows). ( B ) 4A4+/GFAP+ metaphase cell in the E65 macaque occipital cortex. During metaphase GFAP encircled the nucleus in a relatively uniform pattern. The “GFAP cap” (white arrowheads) often appeared on one side of the condensed chromatin during metaphase. ( C ) 4A4+/GFAP+ anaphase cell in the E65 macaque occipital cortex. GFAP-IR surrounded the dividing chromosomes and was also present between the sister chromatids during anaphase. ( D ) 4A4+/GFAP+ telophase cell from E80 macaque occipital cortex. GFAP-IR surrounded the dividing sister chromatids of telophase cells and strong GFAP-IR was present in the cleavage furrow. Scale bar = 5 µm.