Figure 13. The majority of GFAP-negative mitoses in the prenatal macaque neocortex are Mash1/ASCL1+.
Nevertheless, approximately 94% of ASCL1+ mitotic cells in the prenatal macaque cerebral cortex express GFAP. ( A, B ) Examples of ASCL1+/GFAP+ mitotic cells in the E80 macaque occipital cortex. Sections were immunostained for PH3 (blue), ASCL1 (green) and GFAP (red). ( A, B ) Examples of PH3+/ASCL1+/GFAP+ mitotic cells located in the outer subventricular zone. Inset panels below show immunoreactivity for each individual antibody. ( C ) An example of a PH3+/ASCL1+ mitotic cell that does not express GFAP in the subventricular zone. Scale bars = 10 µm.