Table 3. Summary of humoral and cell-mediated responses against MAP according to histopathological findings.

		% MAP detection		ELISA		IGRA
Histopathology	n	Culture+	rtPCR+	%Pos	AB (Mean OD±SE)	%PPDAV+	%PPDBOV+	PBS(Mean OD±SE)	PPDAV(Mean OD±SE)	PPDBOV(Mean OD±SE)
No PTB lesions	157	3.82	17.20	1.27	0.149±0.010	23.57	28.66	0.128±0.012	0.175±0.015	0.197±0.021
PTB lesions	176	22.16	38.64	10.23***	0.358±0.049***	40.34**	15.34	0.144±0.019	0.256±0.025**	0.203±0.020
Focal	152	13.16	29.61	1.97	0.170±0.016	36.84	17.11	0.147±0.022	0.235±0.026	0.198±0.021
Multifocal	12	58.33	91.67	33.33	0.927±0.308	58.33	0.00	0.088±0.017	0.310±0.129	0.166±0.072
Diffuse	12	100.00	100.00	91.67	2.175±0.247	66.67	8.33	0.161±0.037	0.476±0.097	0.302±0.073
-D.multibacillary	7	100.00	100.00	100.00	2.440±0.149	85.71	0.00	0.120±0.019	0.546±0.141	0.306±0.105
-D.intermediate	4	100.00	100.00	75.00	2.028±0.643	50.00	0.00	0.150±0.060	0.298±0.127	0.196±0.047
-D.lymphocytic	1	100.00	100.00	100.00	0.906	0.00	100.00	0.489	0.694	0.700
Total/Mean	333	13.51	28.53	6.01		32.43	21.62

MAP presence was assessed by tissue culture and rtPCR. Paratuberculosis specific antibodies detected by ELISA (AB) and IFN-γ release assay (IGRA) after blank stimulation (PBS) and stimulation with avian (PPD_AV) and bovine antigens (PPD_BOV) are shown as mean optical density (OD) values and standard error (SE). Statistical significance (Student t-test): *p<0.05; ** p<0.01; *** p<0.001.