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. 2013 May 28;8(5):e63787. doi: 10.1371/journal.pone.0063787

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© 2013 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The recombinant Sp-SPH protein was purified by using Ni2+ affinity chromatography via a 6× His tag as described above. Lane M, molecular weight marker; lane 1, purified Sp-SPH protein; lane 2, cultured medium from pPIC9K/Sp-SPH recombinant clone induced by methonal before protein purification.