Figure 3. Analysis of the binding affinity between recombinant Sp-SPH protein and the bacterial or fungal associated molecules.

The binding affinity of Sp-SPH protein to LPS, PGN or β-1, 3-glucan was tested by ELISA. Absorbance of each well was measured at 450 nm with a Multifunctional microplate reader (GENios). The binding parameters, apparent dissociation constant Kd, and the maximum binding (Amax), were determined by non-linearly fitting as A  =  Amax [L]/(Kd+ [L]). Diamond:lipopolysaccharide (LPS); Square: peptidoglycan (PGN); Triangle: β-1, 3-glucan. The data were representative of the average value of four repeated experiments. Bars indicated mean ± S.E. (n = 4).