Figure 4. Determination of hemocyte adhesion activity of the mud crab mediated by Sp-SPH recombinant protein.

Different concentrations of Sp-SPH recombinant protein were used for coating the ELISA plate followed by addition of crab hemocyte suspension. After washing, the cell adhesion was assessed by measuring the OD_595nm value with Multifunctional microplate reader (GENios). *: Significant differences in hemocyte adhesion of Sp-SPH protein treated samples compared to that of non-Sp-SPH protein control (paired t-test, P<0.05). This experiment was repeated for four times. The results were shown as means ± standards errors (n = 4).