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. 2013 May 28;8(5):e63787. doi: 10.1371/journal.pone.0063787

Figure 5. Phenoloxidase activity enhanced by Sp-SPH protein in the HLS of mud crab.

Figure 5

PGN, Sp-SPH, PGN/Sp-SPH, PGN/Sp-ALF2, or PGN/BSA was added to the mud crab HLS, respectively. The PO activity of HLS was then determined by using L-dopa as substrate and defined as U/mL. Significant difference in PO activity was marked as stars (paired t -test, P<0.05). HLS: hemocyte lysate supernatant; PGN: peptidoglycan+HLS; SPH: Sp-SPH+HLS; PGN+SPH: peptidoglycan+ Sp-SPH protein+HLS; PGN+ALF2: peptidoglycan+antilipopolysaccharide factor2+HLS; PGN+BSA:peptidoglycan+bovine serum albumin+HLS. This experiment was repeated three times and the data represented means of triplicates. Bars indicated mean ± S.E. (n = 3).