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. 2013 May 28;8(5):e63787. doi: 10.1371/journal.pone.0063787

Figure 6. Cumulative mortality of mud crab challenged with bacteria pre-coated with Sp-SPH protein.

Figure 6

Crabs were challenged with A. hydrophila, V. parahemolyticus or V. alginolyiicus pre-coated with Sp-SPH protein as described above. Ten animals were used for each group. The mortality was recorded hourly. The cumulative mortality of crabs, in which the bacteria were coated with Sp-SPH protein (square, SPH), was compared with that of animals treated with “PBS-coated” bacteria (diamond, PBS). A: The cumulative mortality caused by A. hydrophila; B: The cumulative mortality caused by V. parahemolyticus; C: The cumulative mortality caused by V. alginolyiicus. This experiment was repeated three times and the data represented means of triplicates. Bars indicated mean ± S.E. (n = 3).