Figure 2. Retrograde delivery of intrapleural AAV7-TrkB.FL-FLAG to phrenic motoneurons, detected by FLAG expression in ventral horn.
The cervical ventral horn region containing phrenic motoneurons was isolated and analyzed by Western blotting. Top: representative Western blot of FLAG (∼49 kDa) and actin (∼42 kDa) protein expression in the cervical ventral horn region from animals with C2 spinal cord hemisection (SH) performed at 3 weeks after intrapleural AAV7-TrkB.FL-FLAG treatment (SH+AAV7; n = 3). This is compared to that of control rats with no treatment or SH (CTL; n = 3). Bottom: relative expression (mean ± SE) of FLAG compared to actin. Western blot run in duplicate, left and right sides of each animal evaluated independently (bilateral AAV injection).