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. 2013 May 28;8(5):e64755. doi: 10.1371/journal.pone.0064755

Figure 4. Representative raw diaphragm EMG recordings and root mean squared (RMS) EMG tracings after SH.

Figure 4

Untreated SH animals (SH; n = 8) and SH animals treated with intrapleural AAV7-TrkB.FL (SH+AAV7; n = 11) were chronically monitored using EMG recordings obtained via implanted diaphragm electrodes during eupnea. EMG was recorded prior to (pre-SH) and at 3 days (SH 3D) and 14 days post-SH (SH 14D). Diaphragm EMG activity was absent in all animals at SH 3D, confirming complete interruption of descending ipsilateral drive to phrenic motoneurons and resulting diaphragm muscle paralysis. Both ipsilateral and contralateral diaphragm EMG activity occurred in bursts, reflecting rhythmic inspiration in the anesthetized animals. Contralateral diaphragm RMS EMG amplitude increased at SH 14D in both untreated SH and AAV7-TrkB.FL treated SH animals compared to pre-SH EMG activity. In both of the groups, there was a lack of baseline, non-rhythmic EMG activity suggestive of spasms or tonic activity in either side of the diaphragm at any time point post-SH. Intrapleural AAV9-TrkB.FL treated animals (n = 3) displayed qualitatively similar results to the untreated SH group and are not shown.