Figure 3. qRT-PCR validation of down-regulated miRNAs in EAC.

The expression of 4 miRNAs (miR-205, miR-133b, miR-203, and miR-31) was evaluated using qRT-PCR in 46 NS, 13 BE, 17 HGD, and 34 EAC tissues. The data are plotted as the FC of expression relative to miR-191 expression. Each data point represents a different patient tissue sample. The horizontal bars indicate the median. Student’s t-test was used for statistical analysis. A value of p≤0.05 was considered statistically significant.