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. 2013 May 24;5(4):412–426.

Figure 4.

Figure 4

Chronic biotin supplementation abolishes adipose SIRT1-mediated beneficial effects on lipid metabolism and energy expenditure. A: Total (left) and protein-bound (right) biotin in epididymal (epi) fat of WTM and AWSM (36-weeks old) evaluated by ELISA and Western blotting, respectively. B: Serum lipid levels of WTM and AWSM (36-weeks old, fasted for 16 hours). C: Lipid measurement in epididymal (epi) fat, liver and skeletal muscle of WTM and AWSM (36-weeks old, fasted for 16 hours). D: Indirect calorimetry analysis of WTM and AWSM (40-weeks old) (light period 0700-1900). *, P<0.05 compared with the corresponding WTM group (n=10).