Figure 6.

Biotin and biotinyl-5’-AMP inhibit SIRT1-mediated deacetylation reactions. A: In vitro deacetylation assay in the absence or presence of 500 μM nicotinamide (NAM), biotin, biotinyl-5’-AMP, biocytin and AMP. B: HPLC chromatograms of acetylated (top) and deacetylated (bottom) histone H3 peptide. C: HPLC chromatograms of negative (top, reaction without SIRT1 enzyme) and positive (bottom, reaction with SIRT1 enzyme) controls. D: HPLC chromatograms of biotinyl-5’-AMP (top) and positive control reaction in the presence of biotinyl-5’-AMP (bottom). Representative HPLC chromatograms are shown. *, P<0.05 compared with positive control reaction (n=8).