Figure A1.

Differences in expression levels for genes involved in nicotianamine and deoxymugeneic acid synthesis (OsSAMS2, OsNAS1, OsNAS3, and OsDMAS1), in the roots, flag leaves, and grains of NFP vs. NTS plants are presented. Values are mean ± standard error of mean, of the three biological replicates. Significant differences between NFP and NTS tissues (roots, flag leaves, grains) under a particular treatment (high Fe or low Fe) or at a particular development stage (milky, dough, or mature) are marked with single (^*p ≤ 0.05) or double asterisks (^**p ≤ 0.01), respectively. The letters R, L, and G represent root, leaf, and grain, respectively. Note the differences in scale at Y-axis for relative expression levels of tested genes.