Figure 3.
IL-2 does not fully induce IFNγ secretion by NK cells isolated following brief exercise. Pre-exercise (white) and post-exercise (black) isolated NK cells were treated with (A) 50 U/mL or (B) 500 U/mL IL-2. After 4 and 24 h treatment the percentage of IFNγ-secreting NK cells was determined by flow cytometry using an IFNγ capture assay. Data shown represent the mean% ± SD of seven different donors. (C) After 48 h of IL-2 treatment, the amount of IFNγ produced by NK cell cultures (1 × 105 cells/well) was measured by ELISA. Data represent the mean pg/culture ± SD of five independent experiments with cells from different donors. Significant differences are indicated in the graphs as follows: *p < 0.05, **p < 0.01 (paired two tailed student’s t test). (D) Pre-exercise (white) and post-exercise (black) isolated intracellular IFNγ positive NK cells after overnight incubation with 50 U/mL of IL-2 and 0.5 ng/mL of IL-12 followed by additional 6 h of incubation with or without K562 cells at a NK to K562ratio of 5:1 in the presence of GolgiStop. Data represent the mean% ± SD of 5 independent experiments with cells from different donors.