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. 2013 May 29;3:1911. doi: 10.1038/srep01911

Figure 2. Identification of genes modified with H3K27me3 in SCLC cell lines.

Figure 2

(a) ChIP-seq of H3K27me3 in SAEC and SCLC cell lines. Genes not modified with H3K27me3 in either SAEC or SCLC cell lines (GAPDH), modified both in SAEC and SCLC cell lines (ADAM33), and not modified in SAEC but in SCLC cell lines (ITGB4), were representatively shown. Brown bars, regions for ChIP-PCR. (b) The results of ChIP-seq in GAPDH, ADAM33 and ITGB4 were validated by real-time PCR using ChIP samples that were obtained in additionally performed ChIP experiments. (c) Expression of H3K27me3(+) genes was significantly lower than H3K27me3(−) genes in each sample (*P < 10−20, t-test). (d) PRC2-target genes in ES cells28 occupied 3.5% among all the genes (left). Among genes with H3K27me3 in SAEC only, in SAEC and 1 SCLC cell line, and in SAEC and 2–3 SCLC cell lines (center), PRC2-target genes in ES cells occupied 14.3%, 23.8%, and 27.0%, respectively, indicating significant overlap (*P < 10−30, P < 10−30, P < 10−30, Fisher's exact test.). Genes with H3K27me3 in 1 SCLC cell line or 2–3 SCLC cell lines but not in SAEC (right), however, were not significantly overlapped with PRC2-target genes in ES cells, 3.2% (P = 0.66) or 3.8% (P = 0.65), respectively.