Figure 5. Introduction of JUB and PTRF in SCLC cell line.

JUB or PTRF cDNA with V5-tag was introduced in SCLC cell line DMS53 by lentivirus infection. (a) Expression was confirmed by western blotting. Blots were cropped in the figure, but the gels were run under the same experimental conditions, and full-length blots are presented in Supplementary Fig. S3. (b) JUB and PTRF expression by immunofluorescence. Introduced JUB was mainly observed at plasma membrane, and introduced PTRF was mainly observed in the cytoplasm. (c) Growth curve analyzed by WST-8 assay. PTRF-introduced cells showed similar growth to mock cells infected with empty lentivirus, whereas JUB-introduced cells showed suppressed growth significantly (*P < 0.05, t-test). Bars, standard deviation in triplicated analysis.