Figure 8.

Shot regulates MT polymerization in primary neurons. A–D, Endogenous EB1 (A, B) and transgenic EB1::GFP (C, D) can be seen as comets (arrows) in wild-type (left) and shot^−/− mutant neurons (right) alike; to generate the images C and D which illustrate examples of growing MTs, frames from time laps of EB1::GFP-expressing neurons were color-coded alternating in green and magenta and then merged together. E, F, Measurements of the life-time and velocity of EB1::GFP comets in wild-type (black) and shot^−/− mutant neurons (dark gray), and in mutant neurons with targeted expression of Shot constructs (light gray), all normalized to wild-type. The 100% life-time equates to 4.31 frames ± 0.13 SEM (= 17.3 ± 0.52 s) and 100% velocity to 0.156 μm/s ± 0.003 SEM. Statistics were performed using Kruskal–Wallis one-way ANOVA on Ranks (H = 31.987, 3 degrees of freedom, p < 0.001 in F; H = 70.828, 3 degrees of freedom, p < 0.001 in G) and Mann–Whitney rank sum test (*indicates p ≤ 0.004; ns, not significant p > 0.05). Scale bar (in A) A, B, 8 μm; C, D, 4 μm.