Figure 7.

Yeast cells expressing Msg on the cell surface adhere to A549 epithelial cells. Cells transformed with msg construct 3 expressed in pESC-URA or vector alone were stained with carboxyfluorescein diacetate, succinimidyl ester (CFSE) and then tested for their ability to adhere to cultured A549 epithelial cells. A, Fluorescence microscopic analysis demonstrates that most of the yeast cells visible under bright light are stained with CFSE. B, Yeast cells expressing Msg show increased adherence to epithelial cells. After 1 hour of incubation with CSFE-labeled yeast, followed by washing, epithelial cells and adherent yeast were scraped, and fluorescence was quantitated with a fluorometer. Results are the mean of 2 experiments. The error bar represents the SD. Although the results are not significant, in both experiments yeast cells expressing Msg showed an approximately 9-fold higher fluorescence as compared to those transformed with vector alone.