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Nuclear Nrf2 translocation by Rg3. Nuclear fractions were prepared from H4IIE cells incubated with Rg3 (1–10 μg/mL) for 8 h. Nuclear Nrf2 and Lamin A were immunoblotted with their respective antibodies. Lamin A was used as the loading control. The graph of multiple analyses of repeated experiments was assessed by scanning densitometry of Nrf2. Each value represents the mean ± S.E. (*P < 0.05, **P < 0.01, significantly different from control).
