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. 2013 Jun;2(3):212–216. doi: 10.1089/biores.2013.0008

FIG. 2.

FIG. 2.

Microtubule-associated protein 2 (MAP2) immunofluorescence and intermediate filament gene expression in SH-SY5Y cells treated with stretch and RA. (A) MAP2 immunofluorescence with a scale bar of 50 μm. A 20× objective lens was used (eyepiece with 10×). (B, C) Gene expressions specific to neuronal intermediate filament (neurofilament light chain [NFL], nestin) and astrocytic intermediate filament (glial fibrillary acidic protein [GFAP]). Results from reverse-transcription polymerase chain reaction (RT-PCR) are presented with GAPDH as a loading control and by comparing with non–RA-treated, static control set at 1. NFL: sense ACC TCC TCA ACG TGA AGA TGG CTT, antisense ACT CTT CCT TGG CAGC TTC TTC CT; nestin: sense GCC CTG ACC ACT CCA GTT TA, antisense GGA GTC CTG GAT TTC CTT CC; GFAP: sense ACC AGG ACC TGC TCA ATG TC, antisense ATC TCC ACG GTC TTC ACC AC; GAPDH: sense CAT GAC CAC AGT CCA TGC CAT CAC T, antisense TGA GGT CCA CCA CCC TGT TGC TGT A. Comparisons with non–RA-treated, static control are shown with *p<0.05 (n=3). Other comparisons did not reach statistical significance.