FIG. 2.

EGF and bFGF affect the pluripotency of ASCs. Immunofluorescent staining and bright-field micrographs (BF) of ASCs after 20 days of culture in UN or EF medium. (a and b) Expression of Oct4 in ASCs cultured in UN medium (a) or EF medium (b). (c and d) Expression of Sox2 in ASCs cultured in UN medium (c) or EF medium (d). Scale bars, 20 μm. (e and f) Analysis of stemness markers in undifferentiated ASCs cultured in EF medium on three time points, D10, D20, and D30 by RT-PCR (e) and qRT-PCR (f). Gapdh was used as a loading control. (*) p<0.05; (**) p<0.01.