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. 2013 Jun;15(3):224–232. doi: 10.1089/cell.2012.0077

FIG. 3.

FIG. 3.

FIG. 3.

EGF and bFGF reduce ASC osteogenic differentiation. (a and b) ALP staining of ASCs cultured for 7 days in UN medium containing osteogenic induction factors (a) or EF medium containing osteogenic induction factors (b). (c and d) Immunofluorescent staining for Ocn in ASCs cultured for 15 days in UN medium containing osteogenic induction factors (c) or EF medium containing osteogenic induction factors (d). Scale bars, 50 μm. Nuclei are stained with Hoechst. (e and f) Expression of osteogenic markers by ASCs cultured in UN medium containing osteogenic induction factors or EF medium containing osteogenic induction factors for 15 days, by RT-PCR (e) and qRT-PCR (f). Gapdh was used as a loading control. Values in f are expressed relative to ASCs cultured UN medium containing osteogenic induction factors.