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Location of the primers used to genotype the wild-type (WT) and knockout (KO) alleles of the CD3ɛ and CD3ζ genes. Schematic map showing the location of the forward (right pointing arrows) and reverse (left pointing arrows) primers designed to specifically amplify the WT and the KO alleles of CD3ɛ (A) and CD3ζ (B) genes.
