Table 1.

Sequence of Polymerase Chain Reaction Primers Used to Genotype the Wild-Type and Knockout Alleles of the CD3_ɛ and CD3ζ Genes

Gene	Allele specificity	Sense	Sequence
CD3ɛ	WT	Forward	5′-TGC AAG GTT CAC AGT CTT GC-3′
	WT	Reverse	5′-TTC CAC GAC AGA CAG AAC TC-3′
	KO	Forward	5′-GGC AGT GAA AGG GAG AAA TC-3′
	KO	Reverse	5′-TCA TAG CCG AAT AGC CTC TC-3′
CD3ζ	WT	Forward	5′-AAT GAT GCC CAT GTG CCA AC-3′
	KO	Forward	5′-AAG AGG CTC TAA GCT GAA GC-3′
	WT, KO	Reverse	5′-ACA AAC TCC CTT GTT GGC TC-3′

WT, wild type; KO, knockout.