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. 2012 Jan 16;16(1):213–233. doi: 10.1017/S1461145711001933

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© CINP 2012

The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution-NonCommercial-ShareAlike licence <http://creativecommons.org/licenses/by-nc-sa/2.5/>. The written permission of Cambridge University Press must be obtained for commercial re-use.

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Fig. 1. — Genetic deletion of P2rx7s in mice leads to an antidepressant-like phenotype in the forced swim test (FST) and tail suspension test (TST) and mood stabilizing-like phenotype in amphetamine-induced hyperlocomotion test (AH), but does not affect basal interleukin (IL)-1β levels in the amygdala. (a) P2rx7^−/− mice failed to develop the depression-like behaviour typical to the FST. The time of immobility is expressed in seconds. * Indicates significant changes from immobility values observed during min 0–5 of the first day, n = 20/group; * p < 0.05. (b) Genetic disruption decreased basal immobility in the TST (n = 9–11, * p < 0.05 vs. P2rx7^+/+, Student's t test). The immobility time is expressed in seconds. The total test period was 360 s. (c) Amphetamine-induced hyperactivity is significantly attenuated in P2rx7^−/− mice. Mice were placed into the open field arena for a 30-min habituation period and then injected with i.p. saline (Sal) or d-amphetamine-sulfate (2.5 mg/kg). Locomotor activity was assessed for 90 min immediately after the injection and expressed as the percentage of Sal-treated mice, n = 9–10, *** p < 0.001 vs. P2rx7^+/+, Student's t test. Drug- and test-naive male homozygous mice (P2rx7^+/+ and P2rx7^−/−, aged 2–3 months) weighing approximately 30 g were used in the experiments. (d) IL-1β protein level in the amygdala of P2rx7^+/+ and P2rx7^−/− mice after Sal and lipopolysaccharide (LPS) treatment. The IL-1β protein level was similar in the amygdalae of Sal-treated P2rx7^+/+ and P2rx7^−/− mice. Injection of LPS (E. coli; 250 μg/kg i.p.) significantly increased the level of IL-1β in the amygdalae of P2rx7^+/+ mice 6 h after treatment. IL-1β protein level was less elevated in the amygdalae of P2rx7^−/− mice in response to systemic endotoxin. The levels of IL-1β were quantified in the supernatants by ELISA. Data are given as the mean level of cytokines±s.e.m., expressed in pg/ml. * Indicates significant differences between Sal- and LPS-treated and between P2rx7^+/+ and P2rx7^−/− mice (n = 4 per group, *** p < 0.001, two-way analysis of variance).