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. 2012 Jan 16;16(1):213–233. doi: 10.1017/S1461145711001933

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© CINP 2012

The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution-NonCommercial-ShareAlike licence <http://creativecommons.org/licenses/by-nc-sa/2.5/>. The written permission of Cambridge University Press must be obtained for commercial re-use.

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Fig. 3. — P2rx7^−/− mice [grey bars in (a) and (b)] respond with a decreased elevation of adrenocorticotropic hormone (ACTH) and corticosterone in the plasma (a, b) and pituitary (c) and increased norepinephrine (NE) level (d) in the amygdala to stress. Hormonal secretion was evaluated after 30 min of restraint. Plasma levels of ACTH (a) and corticosterone (b) were measured by radioimmunoassay of trunk blood (n = 9–10). The resting ACTH and corticosterone levels were not affected by genotype. Restraint significantly increased the level of both hormones, but the elevation of ACTH levels reached the level of significance in P2rx7^+/+ animals (clear bars) only (p = 0.03). (c) The ACTH response to 5 × 10⁻⁸ m corticotropin-releasing hormone (CRH) was studied in vitro (n = 10 per group). Fifteen-min fractions were collected. CRH treatment significantly increased ACTH secretion. This effect was dependent on the genotype and there was a significantly smaller response in P2rx7^−/− mice. C, control; Res, exposed to 30-min restraint. Post-hoc comparisons: * p < 0.05; ** p < 0.01 vs. control; ^#p < 0.05 vs. P2rx7^+/+. (d) The level of NE, dopamine (DA) and 5-HT was analysed by high performance liquid chromatography in the amygdalae of P2rx7^+/+ and P2rx7^−/− mice after 30 min restraint. The results are expressed as pmol/mg protein. * Represents a significant difference between P2rx7^+/+ and P2rx7^−/− animals, n = 10–12/group, * p < 0.05, ** p < 0.01, Student's t test.