Table 2.
Genes that were up-regulated by lipopolysaccharide (LPS) treatment in the mouse amygdala
| Symbol | Gene ID | Gene name (accession number) | Agilent (FC) | Real-time PCR (RQ) | TaqMan ID |
|---|---|---|---|---|---|
| Ifit1 | 15 957 | Interferon-induced protein with tetratricopeptide repeats 1 (NM_008331) | >1000 | 25.77 p < 0.0001 |
Mm00515153_m1 |
| Casp4 | 12 363 | Caspase 4, apoptosis-related cysteine peptidase (NM_007609) | >1000 | 19.24 p = 0.0007 |
Mm00432307_m1 |
| Ccl5 | 20 340 | Chemokine (C—C motif) ligand 5 (NM_013653) | >1000 | 745.12 p = 0.0002 |
Mm01302428_m1 |
| Ccl9 | 20 308 | Chemokine (C—C motif) ligand 9 (NM_011338) | >1000 | 20.22 p = 0.0001 |
Mm00441260_m1 |
| Cxcl10 | 15 945 | Chemokine (C—X—C motif) ligand 10 (NM_021274) | >1000 | 518.39 p = 0.0002 |
Mm99999072_m1 |
| Cxcl16 | 66 102 | Chemokine (C—X—C motif) ligand 16 (NM_023158) | 55.71 | 7.94 p = 0.0007 |
Mm00469712_m1 |
| Gbp2 | 14 469 | Guanylate nucleotide binding protein 2 (NM_010260) | >1000 | 68.13 p = 0.0001 |
Mm00494575_m1 |
| Gpr65 | 14 744 | G-protein coupled receptor 65 (NM_008152) | >1000 | 10.44 p = 0.0021 |
Mm02619732_s1 |
| Igtp | 16 145 | Interferon γ induced GTPase (NM_018738) | >1000 | 10.43 p = 0.0002 |
Mm00497611_m1 |
| Iigp1 | 60 440 | Interferon inducible GTPase 1 (NM_021792) | >1000 | 46.86 p = 0.0001 |
Mm00649928_s1 |
| Il1rn | 16 181 | Interleukin 1 receptor antagonist (NM_001039701) | >1000 | >1000 p = 0.0001 |
Mm01337566_m1 |
| Il4ra | 16 190 | Interleukin 4 receptor, α (NM_001008700) | 23.58 | 6.81 p < 0.0001 |
Mm00439634_m1 |
| Irgm | 15 944 | Immunity-related GTPase family (NM_008326) | >1000 | 21.93 p = 0.0024 |
Mm00492596_m1 |
| Isg20 | 57 444 | Interferon- stimulated protein (NM_020583) | >1000 | 12.29 = 0.0001 |
Mm00469585_m1 |
| Mpa2l | 100 702 | Macrophage activation 2 like (NM_194336) | >1000 | 61.41 p = 0.0001 |
Mm00843395_m1 |
| Saa1 | 20 208 | Serum amyloid A 1 (NM_009117) | >1000 | 34.91 p = 0.0026 |
Mm00656927_g1 |
| Tlr2 | 24 088 | Toll like receptor 2 (NM_0119057) | >1000 | 36.57 p = 0.0004 |
Mm00442346_m1 |
The table shows the comparison of gene expression data from microarray and real-time polymerase chain reaction (PCR) studies. Seventeen genes were selected among the exclusively up-regulated genes by systemic LPS (250 μg/kg i.p.) in the mouse amygdala (P2rx7+/+ and P2rx7−/−) using the selection filters of >2.0-fold change (FC) in the microarray study. Significant differences were calculated by two-way analysis of variance (ANOVA) followed by Benjamini–Hochberg's multiple correction, with p < 0.05. These changes were validated by TaqMan-based quantitative real-time PCR (TLDA). The detected changes in the expression level are expressed in FC in the microarray study and relative quantity (RQ) in the PCR study. The change of all 17 genes was statistically significant (two-way ANOVA, p < 0.05) and showed similarity in direction by both methods. Samples of RNA from the microarray experiment (n = 4) and from an independent experiment (n = 4) were measured for each group on TLDA and the results were evaluated by RealTime StatMiner software. The TaqMan ID gives the product number of Applied Biosystems Gene Expression Assay used for the validation of the corresponding gene.