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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Muscle Res Cell Motil. 2012 Aug 29;33(6):385–394. doi: 10.1007/s10974-012-9316-7

Figure 1. Influence of temperature on actomyosin kinetics and motility in various myosins.

Figure 1

Motility assays were performed at temperatures ranging from 20°C to 35°C, while ATPase assays and ADP-release measurements were performed at 10–35 °C for (A) Chicken Myosin V (MV), (B) Rabbit Skeletal Muscle Myosin II (SK), (C) Gizzard Smooth Muscle Myosin II (SM), and (D) Mouse Non-Muscle Myosin IIA (NM) (all were HMM fragments). The ATPase measurements were performed at a single actin concentration; MV (20 µM), SK (40 µM), SM (40 µM), and NM (20 µM). The ADP release rate constant was examined with dmantADP. The data is plot in Arrhenius format, and the temperature dependence of the ATPase, motility, and ADP release data fit to a linear relationship for comparison. See Table 1 for summary of ATPase activity (s−1), sliding velocity (µm•s−1), and ADP release (s−1) data.