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. 2013 Jun 15;140(12):2632–2642. doi: 10.1242/dev.090829

Fig. 4.

Fig. 4.

Etv2 acts downstream of Fli1 to control Gata2 and adult haemangioblast specification. (A) Expression analysis showing that Etv2 is a major regulator of gene expression in the DLP (arrows). Note that Etv6 and VegfA expression in the DLP is not dependent on Etv2. (B) Analysis of the transcriptional regulation of Etv2 in the DLP. Expression analysis showing that Etv2 expression in the DLP (arrows) is not dependent on Etv6/VEGFA signalling but dependent on Fli1 and Gata2. Note Etv2 ectopic expression in Fli1 morphants (arrowhead). (C) Expression analysis in staged embryos showing that Gata2 expression in the DLP (arrows) is initially independent of Etv2's transcriptional regulation but is controlled by Etv2 after stage 24 of development. All embryos were hybridised as whole mounts and are shown in lateral view, with anterior to the left and dorsal to the top. All embryos are shown at stage 26, unless otherwise indicated. Numbers of embryos represented by each panel, out of the number analysed, are indicated in the top right corner. (D) Diagram summarising an endogenous cell-autonomous and VEGFA-independent genetic cascade required for Scl expression in the DLP. This cascade is initiated by Fli1 and maintained by Etv2.