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Gene targeting in MSCs.
| Don | N | C | S | T | ATF | RTE |
|---|---|---|---|---|---|---|
| 1# | 0.5 | 17 | 9 | 2 | 7.6 | 22.2% |
| 2# | 1.0 | 36 | 23 | 3 | 4.7 | 13.0% |
| 2# | 3.0 | 98 | 50 | 11 | 7.2 | 22.0% |
Don: donor of bone marrow. N: number of cells nucleofected (×106). C: total number of resistant colonies obtained from each experiment. S: number of colonies screened. T: number of colonies screened as targeted recombinants. ATF: absolute targeting frequency (×10−6) = TC/NS. RTE: relative targeting efficiency = T/S. 1#, 2#: bone marrow donors.
