Table 2.
Purification step | Total activity (U) |
Total protein (mg) |
Specific activity (U/mg protein) |
Purification (fold) |
Recovery (%) |
---|---|---|---|---|---|
Culture filtrate | 2571.50 | 28.61 | 89.84 | 1.00 | 100.00 |
Supernatant 90% of (NH4)2SO4 | 2165.01 | 7.42 | 291.78 | 3.25 | 84.19 |
Sephadex G-75 | 1978.10 | 4.32 | 457.89 | 5.10 | 76.92 |